Similar to tyramide signal amplification (TSA), CFP cleavable TSA fluorophores leverage the catalytic activity of horseradish peroxidase (HRP) to enable the covalent deposition and binding of labeled fluorophores onto target proteins or nucleic acid sequences in situ. Our fluorophores are engineered for much higher reactivity than tyramide fluorophores, making the imaging process significantly faster and more robust than conventional TSA-based applications. Unlike conventional TSA fluorophores, CFP fluorescent signals can be cleaved using mild cleavage reagents, enabling ultra-sensitive reiterative staining with standard antibodies. Furthermore, CFP Fluor technology is highly versatile and can be seamlessly integrated into protocols requiring HRP-based detection, including immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), in situ hybridization (ISH), and proximity ligation assays (PLA).